Fluorescence measurement method, fluorescence measuring device and sample evaluation apparatus using the same

摘要

PROBLEM TO BE SOLVED: To provide a fluorometry, fluorometer, and sample evaluating apparatus for using the same, capable of accurately and efficiently computing waveform data, etc., regardless of the fluctuation in the time waveform of fluorescence.;SOLUTION: A sample S is irradiated with a pulsating pumping light supplied from a pumping source 1, and fluorescence while occurs at the sample S is detected by an optical detector 5 via an optical focusing system 3 and a spectroscope 4. Data analysis is performed simultaneously on the time waveform of the fluorescence at the data processing part 62 of a control device 6, to computer physical quantities such as waveform data and a florescence lifetime. At this point, the time waveform of the pumping light is fixed and arranged to a time base used for the data analysis, and a plurality of fitting commutations are performed as moving the time waveform of the excitation light and a fitting range from an initial location before the pumping light peak toward a completing location after the pumping light peak, to select optimal measurement waveform data according to prescribed selection criterion.;COPYRIGHT: (C)2001,JPO