ENHANCED PROTEIN AGGREGATE REMOVAL BY MIXED MODE CHROMATOGRAPHY ON HYDROPHOBIC INTERACTION MEDIA IN THE PRESENCE OF PROTEIN-EXCLUDED ZWITTERIONS

摘要

This invention relates to methods for enhancing purification of proteins such as monoclonal antibodies by chromatography on carboxyl group-containing HIC supports in the presence of zwitterions that are excluded from protein surfaces. In certain embodiments, the invention may permit more effective separation of non-aggregated protein from aggregated protein.