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MEDIA CONDITIONING FOR IMPROVING GENE DELIVERY EFFICIENCY TO DIFFERENTIATING EMBRYONIC STEM CELLS
MEDIA CONDITIONING FOR IMPROVING GENE DELIVERY EFFICIENCY TO DIFFERENTIATING EMBRYONIC STEM CELLS
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机译:用于提高基因传递效率以分化胚胎干细胞的培养基条件
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摘要
The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from ˜1.4% to ˜3.7% of the population.
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