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METHOD AND DEVICE FOR RAPID DETECTION OF MICROORGANISMS BY CHANGING THE SHAPE OF MICRO-COLONIES IN MICRO-CHANNELS
METHOD AND DEVICE FOR RAPID DETECTION OF MICROORGANISMS BY CHANGING THE SHAPE OF MICRO-COLONIES IN MICRO-CHANNELS
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机译:通过改变微通道中微生物菌落的形状快速检测微生物的方法和装置
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摘要
This invention describes a method of rapid detection of micro-colonies of microorganisms by changing their shape from a regular semi-sphere to a long and thin cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides, and attached to a filter from one side. A micro-channel plate houses a multiplicity of micro-channels (diameter of each channel=1-20 μm, and length 100-1000 μm). The micro-channel plate with cells trapped on the surface of the filter is attached to a nutrient media agar block. Cells produce micro-colonies of a long and thin shape according the shape of the micro-channel. The growth of microorganisms in the micro-channels permits a change in the number of cells to accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thus detection and counting of cells can be accomplished in a rapid fashion. The light absorbance can be enhanced by additional coloration of micro-colonies by cell dyes or artificial chromogenic or fluorogenic substrates.
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