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Method for detecting and/or identifying bacteria of the genus Staphylococcus

机译：检测和/或鉴定葡萄球菌属细菌的方法

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摘要

The present invention relates to a method for detecting and/or identifying bacteria of the genus Staphylococcus, comprising the following steps:A. the nucleic acid material of the bacteria of the genus Staphylococcus is extracted,B. at least one target sequence of the nucleic acid material of the bacteria of the genus Staphylococcus is amplified using at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 1 and/or at least one amplification primer comprising at least 10 nucleotide motifs of SEQ ID No. 2, in order to obtain amplicons of the target sequence,C. the presence of bacteria of the genus Staphylococcus is determined by detecting said amplicons.

机译：本发明涉及一种检测和/或鉴定葡萄球菌属细菌的方法，包括以下步骤： A。提取葡萄球菌属细菌的核酸材料， B。使用至少一种扩增引物扩增葡萄球菌细菌的核酸材料的至少一个靶序列，所述引物包含SEQ ID No.1的至少10个核苷酸基序和/或至少一个为了获得靶序列的扩增子，扩增引物包含SEQ ID No.2的至少10个核苷酸基序， C。通过检测所述扩增子来确定葡萄球菌细菌的存在。 展开▼

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公开/公告号US7659059B2

专利类型

公开/公告日2010-02-09

原文格式PDF

申请/专利权人 ALAIN TROESCH;BRUNO LACROIX;CORINNE JAY;
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申请/专利号US20040560433

发明设计人 CORINNE JAY;ALAIN TROESCH;BRUNO LACROIX;
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申请日2004-07-08

分类号C12Q1/68;

国家 US

入库时间 2022-08-21 18:47:50

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1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

2. 一种简单的同时鉴定和检测产超广谱β内酰胺酶细菌的方法 [C] . 胡付品 ,叶信予 ,朱德妹 . 第七次全国临床微生物学术年会暨第三届两岸三地临床微生物与感染病学术论坛 . 2007

3. 葡萄球菌属的鉴定及分类方法研究 [A] . 郭新梅 . 2006