Methods for modifying the isoelectric Point of Antibodies through the substitution of amino acids in the complementarity Determining Region (CDR)

摘要

A method of changing the plasma electric point of an antibody while maintaining its antigen alliance activity, including changing the CDR of at least one amino acid residue exposed on the surface of a defined complementary region. A multi-purpose antibody is also provided, including the first polypeptide and the second polypeptide, which have different isotopic points. The method for purifying the multi-purpose antibody includes changing the plasma electric point and improving the plasma pharmacology in the antibody plasma. The methods include: Change the electric point of the plasma. Besides,The antibody provided has a modified plasma electric point, including the pharmaceutical component of the antibody as the active component, and the method of producing the antibody and the compound.