首页> 外国专利> Morphologically normal and Fertile plants, comprising a complete modified EPSPS gene

Morphologically normal and Fertile plants, comprising a complete modified EPSPS gene

机译:形态正常和能育的植物,包含完整的修饰EPSPS基因

摘要

A Plant material that has been transformed with a polynucleotide comprising the following components in the 5 'to 3' Direction of Transcription: (i) at least one Enhancer region where the transcription Enhancer that is upstream of the transcription start of the STRI ng + Garter Set Cia from which is obtained the Enhancer and the Enhancer per se does not function as a promoterWhether in the sequence in which falls in heterologamente endogenously or when present as part of a construction, (ii) The EPSPS Gene Promoter from Rice, (iii) The Rice genomic Sequence that encodes a chloroplast Transit Peptide of EPSPS of Rice (iv) the genomic Sequence that encodes the Rice EPSPS, (V) a Transcription Terminator.Where the sequence that encodes the Rice EPSPS is modified in a first position is mutated so that the residue at this position is instead of THR ile and one second position is mutated so that the residue at this position is to be in place of Pro,Where mutations are introduced in the sequences of EPSPS that comprise the following gnagtamrpltaav conserved region in the wild-type Enzyme so that the sequence is read or modified gnagiamrsltaav; which has been transformed with a Vector comprising the polynucleotide described above ; or has been, or is,In addition, transformed with a polynucleotide comprising regions that encode Proteins capable of conferring on a Plant material containing one of following agronomically desirable traits, resistance to insects, fungi, viruses, bacteria, nematodes, stress, desiccation, and H Erbicidas; and morphologically normal and Fertile plants, complete.
机译:已经在5'至3'转录方向上用多核苷酸转化的植物材料,该多核苷酸包含以下成分:(i)至少一个增强子区域,其中STRI ng + Garter转录起点上游的转录增强子从中获得增强子和增强子本身的Cia不能起启动子的作用,无论是内源异源落入序列还是作为构建体的一部分存在;(ii)水稻的EPSPS基因启动子,(iii)编码水稻EPSPS叶绿体转运肽的水稻基因组序列(iv)编码水稻EPSPS的基因组序列,(V)转录终止子,其中编码水稻EPSPS的序列在第一位置被修饰,因此在这个位置的残基代替了THR突变,并且突变了一个第二个位置,以便该位置的残基代替Pro,在序列中引入了突变在野生型酶中包含以下gnagtamrpltaav保守区的EPSPS,因此该序列被读取或修饰了gnagiamrsltaav;用包含上述多核苷酸的载体进行了转化;或已(或已)另外用包含编码蛋白质的区域的多核苷酸进行转化,该蛋白质能够赋予植物材料,该材料具有以下农学上所需的性状之一,对昆虫,真菌,病毒,细菌,线虫,胁迫,干燥,和H Erbicidas;形态正常和肥沃的植物,完整。

著录项

  • 公开/公告号AR070608A2

    专利类型

  • 公开/公告日2010-04-21

    原文格式PDF

  • 申请/专利权人 SYNGENTA LIMITED;

    申请/专利号AR2009P100472

  • 发明设计人

    申请日2009-02-11

  • 分类号A01H5/00;A01H5/10;C12N15/29;C12N15/82;

  • 国家 AR

  • 入库时间 2022-08-21 18:47:31

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