Fluorescence based Assay to identify compounds that modulate the direct mode of the sodium / calcium Exchanger

摘要

The transporters are a family of Emergent target with enormous potential, which provides scientific and economic opportunities. The Sodium / calcium Exchanger is an important mechanism to remove Ca2 + of different cells. In the heart, extrudes Ca2 + that has entered through the channels of Ca2 + to initiate contraction, while the Na + into the cells of the heart.It is of considerable interest to identify compounds that modulate the activity of the sodium / calcium Exchanger. This application relates to a fluorescence based assay for Detecting compounds u00f4modo modulators (directou00f6 of NCX and NCKX exchangers, Human Gene Family of Na + / Ca2 +).Moreover, relates to a Kit of parts comprising cells that Express a Sodium / calcium Exchanger and the use of the Kit components to test the activity of a compound as an agonist or antagonist Sodium / calcium Exchanger.