use of metalloproteinases (typ) tissue inhibitor linked to glycosylphosphatidylinositol (gpi) fixations for skin lesion treatment

摘要

The present invention relates to fusion constructs of glycosylphosphatidylinositol (GPI) fixed tissue inhibitors of metalloproteinases (TIMPs) and their use for cancer treatment and regenerative medicine. By this approach, GPI-fixed TIMP proteins are incorporated into the surface membrane of tumor cells and make tumor cells sensitive to FAS-induced apoptosis. In addition, the fusion constructs of the present invention are effective agents useful in curing applications. In one embodiment, TIMP is mucin bound followed by GPI to increase surface presentation. The use of GPI to bind TIMP makes the resulting fusion protein particularly useful as an anti-cancer agent for cancer treatment, and in particular any residual cancer following incomplete surgical resection of primary tumors in an individual.