The process for cultivation, isolation and separation of bacteria Corynebacterium pseudotuberculosis

摘要

The process for cultivation, isolation and separation of bacteria Corynebacterium pseudotuberculosis. It is formed from the surface of the abscesses, surgical collection of nodes located in the regions of lymph nodes pre entrapment, pre popliteal and crural, where he was made a trichotomy in place followed by antisepsis using alcohol 70 and iodine solution.The surgical instruments previously autoclaved and packed up to the time of the collection. After the incision of the skin, nodules were removed entirely, preserving the outer fibrous capsule, and immediately packed in sterile containers tightly closed.Then there was a suture of skin by using product bactericidal and wound healing, and the pots conducted way chilled to the laboratory for analysis.The insulation is carried out from the samples taken from the inside of the nodes with the help of the strap of drigalki sown in Petri dishes containing culture medium, solid, in the environment of biological safety cabinet, and then incubated for 48 hours at 37 u00b0 C.