The diagnostic method specific species of Brucella ovis and diagnostic kit for the detection of Brucella ovis by polymerase chain reaction (PCR)

摘要

The diagnostic method specific species of Brucella ovis and diagnostic kit for the detection of Brucella ovis by polymerase chain reaction (PCR).The present invention relates to a diagnostic method species specific and high sensitivity for the detection of the presence of BrucelIa ovis, exclusively based on polymerase chain reaction (per) simple, that is, through the use of a single pair of oligonucleotides The set of selected primers presented in this invention.The invention also allows the use of more than one pair of primers, and also includes a diagnostic kit for the detection of in vitro B. ovis, so use a composition (a) containing at least one of the pairs of oligonucleotideos presented here and the own The composition for the realization of per in the kit.