u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u044f u043fu043bu0430u0437u043cu0438u0434u0430 pPHINS21, coded fusion protein with u043fu0440u043eu0438u043du0441u0443u043bu0438u043du043eu043c rightsa strain of bacteria ESCHERICHIA COLI JM109 / PHINS21 - a producer of hybrid protein u043fu0440u043eu0438u043du0441u0443u043bu0438u043du043eu043c rights and way of u043fu0440u043eu0438u043du0441u0443u043bu0438u043du0430 rights

摘要

the invention belongs to the field of biotechnology, namely receivable with u043fu0440u043eu0438u043du0441u0443u043bu0438u043du0430 rights, and may be used in medicine.the dna construct u043fu043bu0430u0437u043cu0438u0434u043du0443u044e pHINS21, defining the fusion hybrid protein, which u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du044bu0435 sequence u043bu0438u0434u0435u0440u043du043eu0433u043e u043fu0435u043fu0442u0438u0434u0430,an n - angle of gamma interferon, and u043fu0440u043eu0438u043du0441u0443u043bu0438u043du0430 rights connected u043fu0435u043fu0442u0438u0434u043du044bu043c u043bu0438u043du043au0435u0440u043eu043c containing site u044du043du0442u0435u0440u043eu043au0438 fission u043du0430u0437u044b.human proinsulin derived method, including the cultivation of strain - u043fu0440u043eu0434u0443u0446u0435u043du0442u0430 Escherichiacoli JM109 / pHINS21, the u043fu043bu0430u0437u043cu0438u0434u0443 pHINS21, the cells incorporate, solution we in the buffer.in u043cu043eu0447u0435u0432u0438u043du0443 and dithiothreitol, u0440u0435u043du0430u0442u0443u0440u0430u0446u0438u044e hybrid protein, purification u0440u0435u043du0430u0442u0443u0440u0438u0440u043eu0432u0430u043du043du043eu0433u043e hybrid protein u0438u043eu043du043eu043eu0431u043cu0435u043du043du043eu0439 u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439, u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0435 u0440u0430u0441u0449u0435 u043fu043bu0435u043du0438u0435 hybrid protein u044du043du0442u0435u0440u043eu043au0438u043du0430u0437u043eu0439,cleaning u043fu0440u043eu0438u043du0441u0443u043bu0438u043du0430 u043au0430u0442u0438u043eu043du043eu043eu0431u043cu0435u043du043du043eu0439 u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439 followed by highly effective liquid u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439 at reduced phases.the invention simplifies the technological process of u043fu0440u043eu0438u043du0441u0443u043bu0438u043du0430 with man.