BLOOD BASED IMMUNOPROBE ASSAY USING BIOMARKER POLY-ADP RIBOSYLATION OF CELLULAR PROTEIN FOR EARLY DETECTION OF HUMAN CANCER

摘要

This invention relates to a blood based immunoprobe assay using biomarker poly-ADP ribosylation of cellular protein for early detection of human cancer comprising, developing antibody against ADP ribose polymers, subsequently raising polyclonal antibody in a rabbit against the natural heterogeneous mixture of ADP-ribose polymers isolated from mouse spleen cells, developing and standardizing and ELISA based immunoprobe assay using the PAb, alkaline-phosphatase (ALP) or horse radish peroxidase (HRP) conjugated second Ab, and 5-Bromo-4-Chloro-3"-Indolyl Phosphate p-Toluidine (BCIP) + Nitro-Blue Tetrazolium Chloride (NBT) based color developer.