Bifunctional protein anchors comprising a peptidoglycan binding doman fused to an antigen binding domain

摘要

Disclosed is an antigen-loaded immunogenic carrier complex, comprising at least one bifunctional polypeptide attached to an immunogenic carrier, said polypeptide comprising a peptidoglycan binding domain (PBD) through which the polypeptide is attached non-covalently to said carrier, fused to an antigen binding domain (ABD) capable of binding non-covalently an antigen of interest, wherein said PBD comprises an amino acid sequence selected from the group consisting of (i) a LysM domain; (ii) an amino acid sequence retrieved from a homology search in an amino acid sequence database with a LysM domain in the C-terminus of Lactococcus lactis cell wall hydrolase AcmA (said domain herein also referred to as AcmA LysM domain) wherein said retrieved sequence has at least 70% homology to said AcmA LysM domain; and (iii) a sequence showing at least 70% sequence identity to (i); provided that the PBD is capable of attaching to the cell wall of a Gram-positive microorganism; and wherein at least one antigen of interest is bound to said ABD. Further disclosed are methods of providing said antigen loaded immunogenic complex, pharmaceutical compositions and uses thereof.