Procedure detection and in vitro quantification of HIV DNA by quantitative PCR.

摘要

An in vitro method of quantitative detection of DNA of the HIV virus that comprises a double amplification of viral DNA present in the sample using a double quantitative PCR nested, so that a first pair of PCR primers, represented by SEQ ID NO: 1 and SEQ ID NO: 2 which specifically binds to the gag gene at positions 1260 to 1482 used primeraamplificación, and a second pair of PCR primers with a melting temperature (Tm) different from the first pair of primers, joins the gag within the amplified by the first primer pair, characterized fragment gene for amplification and detection of viral DNA is carried out in a single capillary per sample with a reaction mixture containing the two pairs of primers and probes.