Extracellular vesicles of non-pathogenic amoeba useful for transferring a molecule of interest in a eukaryotic cell.

摘要

Method for preparing a vesicle non-pathogenic amoeba that contains one selected from the group consisting of a therapeutic molecule, an agent imaging and a diagnostic agent exogenous molecule of interest, comprising the steps consisting of: a) culturing a non-pathogenic amoeba in a culture medium comprising said exogenous molecule of interest, under conditions sufficient to allow the non-pathogenic amoeba cell to release vesicles, b) recovering a vesicle released by said cell, containing the vesicle said exogenous molecule of interest, with provided that said vesicle is not a Dictyostelium discoideum vesicle containing Hoechst 3334 that