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Method for amplifying genetic information using primers that bind at multiple sites in the genome.

机译:使用结合基因组多个位点的引物扩增遗传信息的方法。

摘要

Procedure for determining the relative frequency of a delimitable partial of a genetic material, being amplified genetic material by polymerase chain reaction, wherein primers are used, which are complementary to each binding sites primers which they are present in the genetic material at several sites, and are in each contiguous case with respect to a target sequence having a predetermined length, and is specific in each case a partial set, so that a product is obtained amplification, which essentially has only a amplified sequences comprising a target sequence having a predetermined length, and is specific to the respective genetic information, which is suitable for detection by hybridization being analyzed amplification product by hybridization experiment for the determination of the frequency relative delimitable partial.
机译:通过聚合酶链反应确定遗传材料可界定部分的相对频率的程序,该遗传材料是通过聚合酶链式反应扩增的遗传材料,其中使用的引物与每个结合位点互补,它们存在于遗传材料中几个位点上,相对于具有预定长度的靶序列在每种情况下都是连续的,并且在每种情况下都是特异性的,从而获得扩增产物,该产物基本上仅具有包含具有预定长度的靶序列的扩增序列,并且针对各自的遗传信息,适合于通过杂交检测被分析的扩增产物,通过杂交实验确定频率相对可界定的部分。

著录项

  • 公开/公告号ES2333715T3

    专利类型

  • 公开/公告日2010-02-26

    原文格式PDF

  • 申请/专利权人 OLYMPUS LIFE SCIENCE RESEARCH EUROPA GMBH;

    申请/专利号ES20030797298T

  • 发明设计人 MANN WOLFGANG;

    申请日2003-09-11

  • 分类号C12Q1/68;

  • 国家 ES

  • 入库时间 2022-08-21 18:43:10

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