Quantifying cartinina levels in dialysis patients.

摘要

A method for quantifying the levels of concentration of free carnitine, total acylcarnitines and total carnitine in plasma samples of dialysis patients, using mass spectrometry cascade, said method comprising: obtaining a plurality of samples of calibration curve containing dialyzed plasma having mixed therein known concentrations of free carnitine; preparing a plurality of patient samples containing plasma collected from a patient and said samples of calibration curve for analysis, which comprises extracting the patient samples and samples of calibration curve with an alcohol solution containing labeled internal standards; analyzing said patient samples to quantify concentrations of free and acylcarnitine carnitine relative to internal to produce patient data patterns and analyzing said samples calibration curve to quantify free carnitine and acylcarnitines in relation to patterns internal to produce calibration curve data; comparing said patient data to said calibration curve data to obtain carnitine concentration data, said data including carnitine concentration free carnitine concentration, acetylcarnitine concentration and total acylcarnitines concentration for each patient sample; and correcting said free carnitine concentration and said acetylcarnitine concentration to obtain the concentration of free carnitine quantized concentration quantified total acylcarnitine, and concentration of total carnitine quantified for each patient sample, in which the correct concentration of free carnitine It takes into account the hydrolysis of acylcarnitines into free carnitine and the correcting acetylcarnitine concentration takes into account the interference of glutamic acid, and wherein the concentration quantified total carnitine is determined by adding said concentration quantified total acylcarnitines and the concentration of quantified free carnitine.