GENOME SEQUENCES OF PRIMERS AND A PROBE FOR THE QUANTIFICATION OF PORCINE ADENOVIRUSES (PAdV)

摘要

The invention relates to a sensitive test for the detection of porcine adenoviruses in environmental samples, water and food. Specifically, the invention relates to a quantitative tool intended for the analysis of porcine adenoviruses as indicators of the presence of porcine contamination. The invention further relates to a kit comprising two sequences of primers and a probe sequence capable of detecting and quantifying the aforementioned virus by means of PCR. The test has been proven to have great specificity since it has not displayed positive results in samples containing human or bovine adenoviruses. In addition, the test detected porcine fecal contamination in samples that were highly diluted and had been collected at a considerable distance from the initial source.