In situ hybridization method for detecting a target nucleic acid.

摘要

A method for detecting a target nucleic acid fragment directly from a specimen obtained from a patient by in situ hybridization comprising the steps: a) depositing a sample of the specimen onto a slide; b) fixing the sample to the slide with fixative comprising methanol and acetic acid in a ratio of 99: 1 to 80:20. c) contacting the nucleic acids of the fixed sample with a nucleic acid probe specific for the labeled target nucleic acid fragment, in conditions appropriate for hybridization; d) remove rinse the probe labeled unhybridized nucleic acid sample; e) staining the rinsed sample with Evans Blue; and f) visually detecting the hybridized probe complex by microscopy, with the presence of the probe complex an indication of the presence of target nucleic acid fragment.