Immobilisation procedure and utilization of supercoiled DNA to analyze DNA repair.

摘要

Procedure immobilizing a supercoiled DNA, characterized by comprising the following steps: - depositing a sample of supercoiled DNA on the surface of a porous polymer film constituted by a thin layer of a three-dimensional matrix comprising chains of one or more polymers and a solvent, wherein the matrix includes interstices that can contain macromolecules such as DNA, and - immobilizing the supercoiled DNA by passive diffusion in said porous polymer film.