DNA EXTRACTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOOD CHIPS, LAMP PRIMER SET FOR BURSAPHELENCHUS XYLOPHILUS, AND DETECTION METHOD FOR BURSAPHELENCHUS XYLOPHILUS FROM WOODCHIPS

摘要

To extract and detect Bursaphelenchus xylophilus DNA withoutisolating Bursaphelenchus xylophilus from a piece of wood takenfrom a tree belonging to the genus Pinus and the like, providedare a method for extracting Bursaphelenchus xylophilus DNA froma collected piece of wood that includes Bursaphelenchus xylophilus,a LAMP primer set including primers that anneal to a specific regionof Bursaphelenchus xylophilus DNA, and a method for detectingBursaphelenchus xylophilus from a piece of wood by amplifying aDNA by a LAMP method using this primer set.