NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (NGAL) PROTEIN ISOFORMS ENRICHED FROM URINE AND RECOMBINANT CHINESE HAMSTER OVARY (CHO) CELLS AND RELATED COMPOSITIONS, ANTIBODIES, ANDMETHODS OF ENRICHMENT, ANALYSIS AND USE

摘要

A composition comprising neutrophil gelatinase-associated lipocalin (NGAL),which has been enriched fromurine, has a molecular weight of about 24.9 kDa to about 25.9 kDa, andcomprises a plurality of isoforms having isoelectric points(pls) ranging from about 5.9 to about 9.1; a composition comprising NGAL,which has been enriched from recombinant Chinesehamster ovary (CHO) cells, has a molecular weight of about 25.9 kDa to about27.9 kDa, and comprises a plurality of isoformshaving pls ranging from about 5.6 to about 9.1; a method of obtaining fromurine a composition comprising a plurality of isoformsof NGAL, which method comprises enriching NGAL in urine without separatingmolecules based on charge; a method of obtainingfrom recombinant CHO cells a composition comprising a plurality of isoforms ofNGAL, which method comprises enrichingNGAL in a composition without separating molecules based on charge; and amethod of analyzing NGAL isoforms enriched fromurine or recombinant CHO cells comprising analyzing an enriched compositioncomprising NGAL isoforms by two-dimensionalelectrophoresis and Western blot.