Sample mounts for microcrystal crystallography

摘要

Sample mounts ( 10 ) for mounting microcrystals of biological macromolecules for X-ray crystallography are prepared by using patterned thin polyimide films ( 12 ) that have curvature imparted thereto, for example, by being attached to a curved outer surface of a small metal rod ( 16 ). The patterned film ( 12 ) preferably includes a tapered tip end ( 24 ) for holding a crystal. Preferably, a small sample aperture is disposed in the film for reception of the crystal. A second, larger aperture can also be provided that is connected to the sample aperture by a drainage channel, allowing removal of excess liquid and easier manipulation in viscous solutions. The curvature imparted to the film ( 12 ) increases the film's rigidity and allows a convenient scoop-like action for retrieving crystals. The polyimide contributes minimally to background and absorption, and can be treated to obtain desired hydrophobicity or hydrophilicity.