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RECOMBINANT ESCHERICHIA COLI FOR PRODUCING RECOMBINANT HUMAN VARIATION INTERFERON-BETA PROTEINS WHEREIN METHIONINE AT THE AMINO END IS REMOVED, AND PREPARATION METHOD THEREOF
RECOMBINANT ESCHERICHIA COLI FOR PRODUCING RECOMBINANT HUMAN VARIATION INTERFERON-BETA PROTEINS WHEREIN METHIONINE AT THE AMINO END IS REMOVED, AND PREPARATION METHOD THEREOF
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机译:用于去除人末端蛋氨酸蛋氨酸的重组人变异干扰素-BET蛋白的重组埃希氏菌属及其制备方法
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摘要
The present invention relates to recombinant Escherichia coli for producing recombinant human variation interferon-beta proteins wherein methionine at the amino end is removed, and to a preparation method thereof, and more particularly, to recombinant Escherichia coli for producing recombinant human variation interferon-beta proteins and to a preparation method thereof, wherein methionine at the amino end is removed by inserting recombinant human variation interferon-beta (rHu IFN-βSer17, IFN-β 1b) genes into pET21a(+) vector to produce recombinant expression vector pET21a(+)IFN-βSer17, inserting methionine aminopeptidase genes into pSTV29 vector to produce recombinant expression vector pSTV29Map, and transforming the two recombinant expression vectors in a single colon bacillus (BL21(DE3)).
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