PRODUCTION OF RECOMBINANT PHOTOSYNTHETIC BACTERIA WHICH PRODUCES MOLECULAR HYDROGEN IN LIGHT INDEPENDENT MANNER AND HYDROGEN EVOLUTION METHOD USING THE STRAIN

摘要

The present invention relates to hydrogen production of a variant from Rhodobacter sphaeroides which expresses foreign enzyme related to anaerobic Hydrogen production. Conventional Rhodobacter sphaeroides wild type strain is capable of producing hydrogen of high efficiency according to the action of nitrogenase under photosynthetic condition, but it has a limitation in producing hydrogen at night where there is no light. In case of ameliorated variant provided in the present invention, even at night without light it is possible for anaerobic hydrogen synthase which is expressed after being introduced from the outside to produce hydrogen, and in case of variant from the the Rhodobacter sphaeroides, it is possible for anaerobic hydrogen synthase which was introduced together with conventional nitrogenase to function simultaneously under photosynthetic condition. This has much more improved hydrogen productivity.