DETERMINATION OF CIRCULATING 1ALPHA, 25 (OH) 2D3 BY ENZYME ELECTRODE

摘要

This invention includes a method of determining the level of 1α,25 (OH)2D3 by the steps of (a) bringing a bio-electrode bearing an immobilized recombinant 1α-hydroxylase into electrical contact with a biological material; (b) determining the current generated at said bio-electrode caused by catalytic turnover of immobilized said recombinant 1α-hydroxylase; and, (c) relating said current to a level of 1α,25(OH)2D3 in said biological material