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DUAL-FLUORESCENT REPORTER CONSTRUCT AND ASSAY FOR MEASURING TRANSLATIONAL RECODING
DUAL-FLUORESCENT REPORTER CONSTRUCT AND ASSAY FOR MEASURING TRANSLATIONAL RECODING
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机译:双荧光报告者构建和评估翻译的方法
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摘要
The invention relates to a translational recoding reporter construct comprising: a first fluorescent protein coding sequence; a second fluorescent protein coding sequence encoding a fluorescent protein different from the first fluorescent protein; and a linker sequence interposed between the first and second fluorescent protein coding sequence; wherein the a first fluorescent protein coding sequence and the second fluorescent protein coding sequence are out-of-frame with respect to each other but are co-expressed as a single fusion polypetpdie upon recoding. The invention also relates to methods of use of the construct in screening modulators of recoding.
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