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ELECTROTRANSFORMATION OF GRAM-POSITIVE, ANAEROBIC, THERMOPHILIC BACTERIA
ELECTROTRANSFORMATION OF GRAM-POSITIVE, ANAEROBIC, THERMOPHILIC BACTERIA
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机译:革兰氏阳性,厌氧,嗜热细菌的电转化
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摘要
The present invention relates to methods for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation and Gram-positive, anaerobic, thermophilic bacteria transformed by the disclosed methods. The methods employ voltage pulsing schemes that decrease arcing such that increased transformation efficiency and cell viability is observed. The present invention is further directed to a method for transforming Gram-positive, anaerobic, thermophilic bacteria via electroporation using recovery/selection temperatures to effect increased transformation efficiency in difficult to transform bacteria.
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