METHODS FOR MEASURING THE INTRACELLULAR QUANTITY OF INTRINSICALLY FLUORESCENT MOLECULES OF INTEREST BY MEANS OF FLOW CYTOMETRY AND USES THEREOF

摘要

The invention relates to methods for measuring the intracellular quantity of an intrinsically fluorescent molecule of interest in cells, comprising the following steps consisting in: measuring the fluorescence of the cells for which the intracellular quantity of the molecule is required, using a flow cytometer parameterised with the emission and absorption spectra of the molecule of interest, said fluorescence value being plotted on a line of correlation between the fluorescence intensity and the intracellular concentration of the molecule of interest; and reading the intracellular quantity of the molecule of interest.