METHOD FOR ENHANCING PRDUCTIVITY OF HUMAN INTERFERONE BETA MONOMER THROUGH CULTURING TRANSFORMED CELL LINE AT LOW TEMPERATURE AND HYPEROSMOSIS

摘要

PURPOSE: A method for enhancing productivity of human interferon beta monomer by reducing aggregation of the human interferon beta is provided.;CONSTITUTION: A productivity of human interferon beta monomer is enhanced by transforming the cell line for expressing human interferon beta and culturing in a medium of hyperosmosis. The cell line is CHO(Chinese hamster ovary), VERO, BHK, HeLa, Cv1, MDCK, 293, 3T3, myeloma, PC12 or WI38 cell. The used cell line is CHO cell in which DHFR gene is deleted. The condition of hyperosmosis is 350~550 mOsm/kg. The medium is serum-free medium. A method for enhancing producitivity of human interferone beta monomer comprises a step of transferring transformed cell line to medium of hyperosmosis to express human interferon beta.;COPYRIGHT KIPO 2010