METHOD FOR SCREENING AMINOACYL-TRNA SYNTHETASE THROUGH NEGATIVE AMBER SUPPRESSION

摘要

PURPOSE: A method for screening ARS(aminoacyl-tRNA synthetase) is provided quickly and sensitively screen ARS with low cost.;CONSTITUTION: A method for screening ARS(aminoacyl-tRNA synthetase) comprises a step of inserting an amber codon to ccdB(control of cell death B) gene to suppress growth of bacteria by expressing ccdB having high citotoxicity when the amber suppression occurs. The ccdB gene is an inhibito of DNA gyrase.;COPYRIGHT KIPO 2010