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REAL-TIME CONTINUOUS DETECTION DEVICE OF ANALYTE BY CONTINUOUSLY RECYCLING REVERSIBLY REACTIVE CAPTURE RECOGNITION COMPONENT, AND ITS METHOD
REAL-TIME CONTINUOUS DETECTION DEVICE OF ANALYTE BY CONTINUOUSLY RECYCLING REVERSIBLY REACTIVE CAPTURE RECOGNITION COMPONENT, AND ITS METHOD
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机译：连续回收可逆反应性捕获识别组分的分析物实时连续检测装置及其方法
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摘要
PURPOSE: A device for detecting an analyte continuously in real time, and its method are provided to measure the concentration gradient of analytes in real time, and to simplify the constituting parts.;CONSTITUTION: A device for detecting an analyte continuously in real time comprises a sample inflow channel, a sample-analysing site, and a sample outflow channel, wherein the sample-analysing site comprises a sensor which detects the signal generated from a reversibly reactive capture recognition component and an analyte in sample-capture recognition component combination. The reversibly reactive capture recognition component has an adherence speed constant (ka) of 1×10^4 ~ 1×10^7 L·mol^-1·sec^-1, and a desorption rate constant (kd) of 1×10^(-5) ~ 1×10^(-2) sec^-1 in case of the reaction of an analyte in sample, and has a high affinity of an equilibrium adhesion constant (KA = ka/kd) of 1×10^6 L/mol or more.;COPYRIGHT KIPO 2010
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机译：目的：提供一种实时连续检测分析物的装置，其方法可以实时测量分析物的浓度梯度，简化组成部分。样品流入通道，样品分析位点和样品流出通道，其中样品分析位点包括传感器，该传感器检测由可逆反应的捕获识别组分和样品捕获识别组分组合中的分析物产生的信号。可逆反应性捕获识别成分的附着速度常数（ka）为1×10 ^ 4〜1×10 ^ 7 L mol -1 sec -1，并且解吸速率常数（kd）为10乘以10。在样品中分析物反应的情况下，^（-5）〜1×10 ^（-2）sec ^ -1，并且具有1×10的平衡粘附常数（KA = ka / kd）的高亲和力^ 6 L / mol或更高;; COPYRIGHT KIPO 2010
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