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Method for producing a caffeic acid to increase the activity of γ-GCS from deulkkaetip, the caffeic acid, and food compositions and pharmaceutical compositions comprising said caffeic acid produced therefrom
Method for producing a caffeic acid to increase the activity of γ-GCS from deulkkaetip, the caffeic acid, and food compositions and pharmaceutical compositions comprising said caffeic acid produced therefrom
A method for producing caffeic acid improving gamma-GCS activity from green perilla leaves is provided to secure an improvement to liver and antioxidant effect at a little cost. A method for producing caffeic acid improving gamma-GCS activity from green perilla leaves comprises: a first step of reflux-extracting dried green perilla leaves in distilled water; a second step of centrifuging the extract to obtain supernate from it; a third step of filtering the supernate and vacuum-concentrating and freeze-drying the filtrate in order to obtain powder; a fourth step of extracting the powder using a plurality of extraction solvents in sequential order; and a fifth step of separating and refining the fractions showing high gamma-GCS activity. In the first step, 100g of the dried green perilla leaves are extracted in 0.8-1.2L of distilled water at 100-110°C for 3-4 hours twice. In the second step, the centrifugation is performed at 4°C and 7500rpm for 25-30 minutes. The extraction solvents are a first, second, third and fourth extraction solvent.
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