ULTRA HIGH SENSITIVITY DETECTION OF C-REACTIVE PROTEIN USING GOLD NANOPARTICLE

摘要

PURPOSE: A method for measuring C-reactive protein using gold nanoparticle is provided to measure ultra highly-sensitive biomarker in a real time at 100 fM level.;CONSTITUTION: A ultra highly-sensitive detection of C-reactive protein comprises: a step of adding reaction buffer to a sensor chip to obtain the number of vibration(F1) at a normal status; a step of adding mixture of streptavidin-coated gold nanoparticle and test sample containing biotinylated C-reactive protein antibody and C-reactive protein on the sensor chip; a step of amplifying signal by binding streptavidin-coated gold nanoparticle to the biotinylated C-reactive protein antibody conjugated with C-reactive protein; and a step of measuring variation of the number of vibration(ΔF = F1 - F2).;COPYRIGHT KIPO 2010