ACETYL XYLAN ESTERASE NFAXE1 WHICH IS DERIVED FROM NEOCALLIMASTIX FRONTALIS

摘要

PURPOSE: An acetyl xylan esterase I(NfAXE1) which is derived from Neocallimastix frontalis is provided to perform hydrolysis of ester bond of acetyl group in acetylated xylan.;CONSTITUTION: An acetyl xylan esterase NfAXE1 which is derived from Neocallimastix frontalis comprises an amino acid of sequence number 2. The NfAXE1 enzyme protein has optimal activity at 40°C and pH 8. The NfAXE1 has a substrate-specificity to p-nitrophenyl acetate, α-naphthyl acetate, acetylated bulkwood xylan or acetylated beaachwood xylan. A gene encoding the NfAXE1 comprises sequence of sequence number 1. A pET28a-NfAXE1 recombinant vector contains NfAXE1 gene.;COPYRIGHT KIPO 2010