METHOD FOR SYNTHESIZING PROSTAGLANDIN E2 SYNTHETASE USING TRNASFORMED E.COLI WHICH EXPRESSES MUTANT MPGES-1

摘要

PURPOSE: A method for synthesizing prostaglanding E2 synthetase using E.coli is provided to massively express mPGES-1 expression vector.;CONSTITUTION: A mutant gene encoding Mpges-1 which is synthetase of prostaglandin E of sequence number 1 is prepared by substituting CGG codon of 40th, 73th, and 122th amino acid, arginine with CGC codon. An expression vector pPGES-2 contains a mutant gene encoding mPGES-1. A method for synthesizing massive mPGES-1 enzyme comprises: a step of inserting a gene encoding a human mPGES-1 enzyme to a E.coli primary expression vector; a step of mutating the mPGES-1 gene in the expression vector to obtain secondary expression vector; a step of inserting the secondary expression vector to E.coli to obtain transformant; and a step of expression mPGES-1 gene from the transformant and isolating and purifying the mPGES-1 enzyme from the transformant.;COPYRIGHT KIPO 2010