首页> 外国专利> TRANSGENIC POTATO IN WHICH METHIONINE CONTENT IS INCREASED USING PERILLA-DERIVED SEED STORAGE PROTEIN GENE AND A METHOD FOR PREPARING THE SAME

TRANSGENIC POTATO IN WHICH METHIONINE CONTENT IS INCREASED USING PERILLA-DERIVED SEED STORAGE PROTEIN GENE AND A METHOD FOR PREPARING THE SAME

机译:利用紫苏来源的种子贮藏蛋白基因提高蛋氨酸含量的转基因马铃薯及其制备方法

摘要

PURPOSE: A transformant having high content of methionine using perilla-derived seed storage protein is provided to specifically overexpress PrLeg in potato tuber and produce crops having rich necessary amino acid content.;CONSTITUTION: An expression vector contains a gene of perilla-derived seed storage protein. A promoter of a gene for tuber-specific expression is Patatin promoter derived from potato. The gene of seed storage protein is nucleic acid encoding PrLeg protein. The expression vector is CaMV35S::PrLeg/pSJ001, or Patatin::PrLeg/pSJpatatin01. A method for preparing a transformant having high content of methionine comprises: a step of preparing the expression vector; a step of transforming plant tissue with the expression vector; and a step of selecting transformant in which the expression vector is introduced.;COPYRIGHT KIPO 2010
机译:目的:利用紫苏来源的种子贮藏蛋白提供高蛋氨酸含量的转化子,以在马铃薯块茎中特异表达PrLeg,并生产出具有必需氨基酸含量的农作物。蛋白。用于块茎特异性表达的基因的启动子是来源于马铃薯的Patatin启动子。种子贮藏蛋白的基因是编码PrLeg蛋白的核酸。表达载体是CaMV35S :: PrLeg / pSJ001或Patatin :: PrLeg / pSJpatatin01。一种蛋氨酸含量高的转化子的制备方法,包括:制备表达载体的步骤;用表达载体转化植物组织的步骤; ;以及选择导入表达载体的转化子的步骤。; COPYRIGHT KIPO 2010

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