MEDIUM PRODUCING CAPSULAR POLYSACCHARIDE FROM STREPTOCOCCUS SP. WITH HIGH YIELD AND A PURIFICATION

摘要

PURPOSE: A medium for producing capsular polysaccharide is provided to optimize medium ingredient and culture condition and to enhance productivity of the polysaccharide.;CONSTITUTION: A medium which produces capsular polysaccharide from streptococcus sp. contains 30g/l of carbon source selected from glucose, fructose, galactose and lactose, 5-30 g/l of yeast extract, and 2-10g/l of sodium chloride. A method for purifying capsular polysaccharide comprises: a step of adding sodium deoxycholate to culture medium of Streptococcus sp. to perform cell lysis; a step of adding chloroform-butanol solution to the cell lysate to obtain supernatant; and a step of adding ethanol to the supernatant to precipitate capsular polysaccharide.;COPYRIGHT KIPO 2010