ANTIBODY WHICH IS ABLE TO RECOGNIZE HYALURONIDASE PH-20 SPECIFICALLY EXPRESSED IN PORCINE TESTIS CELLS AND A METHOD FOR MANUFACTURING THE SAME

摘要

PURPOSE: An antibody which specifically recognizes porcine hyaluronidase PH-20 and a method for manufacturing the same are provided to prevent polyspermy and to enhance isolation efficiency of spematogonia stem cells.;CONSTITUTION: A method for manufacturing an antibody which specifically recognizes porcine hyaluronidase PH-20 comprises: a step of preparing a recombinant protein containing zona pellucida binding domain of porcine hyaluronidase; a step of isolating the recombinant protein and inducing immune reaction by injecting to an animal; a step of isolating antibody which specifically recognizes the porcine hyaluronidase PH-20 from animal serum.;COPYRIGHT KIPO 2010