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STRAIN HAVING DECOMPOSITION ABILITY OF DIESEL ISOLATED FROM OIL-CONTAMINATED SOIL

机译:石油污染土壤中分离出的柴油具有应变分解能力

摘要

PURPOSE: A strain which biologically decomposes diesel is provided to remove diesel form contaminated soil and to ensure decomposition ability.;CONSTITUTION: A Pseudomonas aeruginosa TPH1(KACC 91384P) has diesel decomposition ability. The Pseudomonas aeruginosa TPH1(KACC 91384P) is isolated from contaminated soil. A method for isolating the strain comprises: a step of culturing collected soil in LB medium containing yeast; a step of inoculating 1% of the strain in MSM medium containing 1% of diesel; and performing sub-culturing three times or more; a step of smearing the stain on LB solid medium and separating single colony; a step of inoculating the single colony to MSM medium; and a step of isolating Pseudomonas aeruginosa TPH1.;COPYRIGHT KIPO 2010
机译:目的:提供一种可生物降解柴油的菌株,以去除受污染土壤中的柴油并确保其分解能力。;组成:铜绿假单胞菌TPH1(KACC 91384P)具有柴油分解能力。从污染的土壤中分离出铜绿假单胞菌TPH1(KACC 91384P)。一种分离菌株的方法,包括:在含有酵母的LB培养基中培养收集的土壤的步骤;在含有1%柴油的MSM培养基中接种1%菌株的步骤;进行3次以上的传代培养;在LB固体培养基上涂抹污渍并分离单个菌落的步骤;将单个菌落接种到MSM培养基中的步骤;和分离铜绿假单胞菌TPH1的步骤。; COPYRIGHT KIPO 2010

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