METHOD FOR MEASURING PHOSPHORYLATION IN A BIOCHIP USING RADIATION TLC IMAGE SCANNER

摘要

PURPOSE: A method for measuring phosphorylation in a biochip using a TLC image scanner is provided to shorten detection time and to develop biochip.;CONSTITUTION: A method for measuring phosphorylation comprises: a step of preparing a biochip for measuring phosphorylatiuon, in which kinase-specific substrate peptide is integrated on the surface of a substrate; a step of hybridizing a test sample with radioactive isotope-labeled ATP; a step of treating the hybridized test sample to the biochip to induce phosphorylation; and a step of detecting phosphorylation intensity using TLC image scanner. The substrate of the biochip is coated with amine group, aldehyde group, carboxyl group or thiol group.;COPYRIGHT KIPO 2011