METHOD FOR DETECTING EPIDERMAL GROWTH FACTOR RECEPTOR TYROSINE KINASE INHIBITOR AND A PHARMACEUTICAL COMPOSITION CONTAINING THE SAME FOR TREATING OR PREVENTING CANCER

摘要

PURPOSE: A method for detecting Epidermal Growth Factor Receptor(EGFR) tyrosine kinase inhibitors is provided to suppress EGFR tyrosine kinase activity.;CONSTITUTION: A pharmaceutical composition for preventing or preventing cancer contains a compound having 2-{[6-(5,6-Dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yloxy)-3,4,5-trihydroxy-tetrahydro-pyran-2-carbonyl]-amino}-4-methylsulfanyl-butyric acid methyl ester structure, 6,7-Dihydroxy-4-(o-tolylaminomethyl)-chromen-2-one structure, or N-[3-(3,4-Dihydroxy-phenyl)-acryloyl]-2-hydroxy-benzamide structure. A method for detecting a compound having activity of suppressing EGFR tyrosine kinase comprises: a step of attaching PLC-gamma1 on a protein chip; a step of reacting EGRF tyrosine kinase, ATP, Mg^2+, and single compound library compound pool; a step of reacting antibody with phosphorylated substrate; a step of washing with buffer; and a step of measuring intensity of fluorescence.;COPYRIGHT KIPO 2010