Method for mass propagation of callus and method for mass division of Iris odaesanensis flower utilizing leaf segments of Iris odaesanensis

摘要

The present invention is yellow iris pattern ( Iris odaesanensis ) relates to a method for mass propagation methods and mass differentiation of callus yellow iris pattern, specifically the yellow iris pattern ( Iris Step harvesting the leaf segment of odaesanensis ); A plant growth hormone fragment is excised leaf, sucrose (Sucrose), and contains the agar and adjusted to pH 5.7 ~ 5.8 MS (Murashige & amp; Skoog) deriving the callus was cultured in dark conditions in a medium; The derived callus include plant growth hormones and sucrose (Sucrose) were inoculated in MS liquid medium, adjusted to pH 5.7 ~ 5.8, and then incubated in a shaking incubator of the light conditions comprising: a callus growth; yellow, characterized in that it comprises a mass propagation method, and deriving the shoots from the callus proliferation of callus iris pattern; And deriving from the roots induced shoot; include and optionally sterilizing the leaf segment was excised after the step of extracting the leaf segment; Or a step to purify the differentiated plants on board after the step of inducing root; relates to a method for mass differentiation of yellow iris pattern, characterized in that it further comprises ; yellow when using the present invention Iris patterns ( Iris odaesanensis ) and Korea endemic plants from leaf segments of a given pattern yellow irises endangered species differentiation to simply and easily mass and to be able to contribute to the conservation of genetic resources, yellow iris pattern by using the present invention ( Iris odaesanensis ) new income of the farming town Florist If you develop a species can contribute to increasing income farming town.