JOINT chemotherapy and immunotherapy

摘要

1. A method of treating a tumor in a subject, comprising the step of:! administering to the subject an effective treatment amount of an EGFRvIII peptide and an effective treatment amount of a chemotherapeutic agent causing lymphopenia. ! 2. The method according to claim 1, where the chemotherapeutic agent is an alkylating agent. ! 3. The method according to claim 1, where the EGFRvIII peptide is conjugated with cochlear lymph hemocyanin (KLH). ! 4. The method according to claim 1, further comprising a stage:! administering to the subject an effective amount of GM-CSF as an adjuvant simultaneously with the EGFRvIII peptide. ! 5. The method according to claim 1, where the peptide EGFRvIII has the sequence H-LEEKKGNYVVTDHS-OH (SEQ ID NO: 1). ! 6. The method according to claim 1, where the peptide EGFRvIII has the sequence LEU-GLU-GLU-LYS-LYS-GLY-ASN-TYR-VAL-VAL-THR-ASP-HIS (SEQ ID NO: 2). ! 7. The method according to claim 1, where the EGFRvIII peptide has the sequence LEU-GLU-GLU-LYS-LYS-GLY-ASN-TYR-VAL-VAL-THR-ASP-HIS-CYS (SEQ ID NO: 3) and where KLH conjugated to the residue CYS. ! 8. The method according to claim 7, where the EGFRvIII peptide is conjugated to a KLH heterobifunctional cross-linking agent. ! 9. The method of claim 8, where the heterobifunctional cross-linking agent is sulfosuccimidyl-6- [3 '- (2-pyridyldithio) propionamido] hexanoate. ! 10. The method according to claim 2, where the alkylating agent is temozolomide or a pharmaceutically acceptable salt thereof. ! 11. The method according to claim 1, where the tumor is a malignant glioma. ! 12. The method according to claim 1, where the tumor is an astrocytoma. ! 13. The method according to claim 1, where the chemotherapeutic agent is an analogue of purine. ! 14. The method according to claim 1, where the chemotherapeutic agent is fludarabine and / or 2-chlorodeoxyadenosine. ! 15. The method according to claim 1, where the tumor is a tumor of the lung. ! 16. The method according to claim 1, where the tumor is a breast tumor. ! 17. The method according to claim 1, where