METHOD FOR PRODUCING STEROID DERIVATIVES BY REDUCING OXOSTEROID COMPOUNDS OR OXIDATION OF HYDROXYSTEROID COMPOUNDS USING A HYDROXYSTEROID DYHYDROGENASE

摘要

1. The method of enantioselective enzymatic recovery of compounds having a steroid structure (ABCD), including one or more heteroatoms, one or more double bonds and / or aromatic link in the cyclic structure, and containing at least one oxo group in position 3, 7, 11, 12 or 17 of the steroidal cyclic system or in the α-position of any carbon residue on the steroid skeleton (= oxosteroid compound),! ! moreover, the oxosteroid compound is reduced with hydroxysteroid dehydrogenase in the presence of the cofactor NADH or NADPH, characterized in that! a) the oxosteroid compound is in the reaction mixture at a concentration of ≥50 g / l,! b) the oxidized cofactor NAD or NADP formed by hydroxysteroid dehydrogenase is continuously reduced via oxidation of a secondary alcohol of the general formula RXRYCHOH, where RX, RY are independently hydrogen, branched or unbranched C1-C8-alkyl, and Ctot ≥ 3, or via oxidation C4-C6-cycloalkanol, and! c) additional oxidoreductase / alcohol dehydrogenase is used to oxidize the secondary alcohol of the general formula RXRYCHOH or cycloalkanol. ! 2. The method according to claim 1, characterized in that the compound of general formula I is used as the oxosteroid compound! ! in which mean:! R1 is hydrogen, methyl group, hydroxy group or oxo group! R2 is hydrogen, methyl group, hydroxy group or oxo group! R3 is hydrogen, a hydroxy group, an oxo group or a methyl group,! R4 is hydrogen or a hydroxy group! R5 is hydrogen, the residue is -COR10, where R10 is an unsubstituted or hydroxy-substituted C1-C4-alkyl group or substituted or unsubstituted