METHOD OF OBTAINING L-AMINO ACIDS OF GLUTAMATE FAMILY WITH USE OF A BACTERIUM OF FAMILY ENTEROBACTERIACEAE

摘要

1. Bacterium of the family Enterobacteriaceae - producing L-amino acids, belonging to the family of glutamate, modified so that the activity of glutamine synthetase is increased in said bacteria. ! 2. The bacterium according to claim 1, characterized in that said activity is increased by enhancing the expression glnA gene. ! 3. The bacterium according to claim 2, characterized in that said glnA gene expression enhanced by increasing the number of copies of the gene or modifying an expression control sequence of the gene, resulting in gene expression is enhanced. ! 4. The bacterium according to claim 1, characterized in that the said bacterium belongs to the genus Escherichia. ! 5. The bacterium according to claim 1, characterized in that the said bacterium belongs to genus Pantoea. ! 6.-producing bacteria L-amino acid according to any one of claims 1-5, characterized in that said L-amino acid belonging to the family of glutamate is selected from the group consisting of L-glutamine, L-proline, L-arginine, ornithine and citrulline. ! 7. A method for producing L-amino acids, belonging to the family of glutamate comprising: - cultivating the bacterium according to claim 1 in a culture medium, and! - collecting said L-amino acid from the medium. ! 8. A method according to claim 7, characterized in that said L-amino acid belonging to the family of glutamate is selected from the group consisting of L-glutamine, L-proline, L-arginine, ornithine and citrulline.