THERMOSTABLE DNA-LIGASE OF THERMOCOCCUS AND METHOD FOR PRODUCING THEREOF AND NUCLEOTIDE DNA SEQUENCE, ENCODING THIS DNA-LIGASE

摘要

1. Recombinant thermostable DNA ligase Thermococcus sp.1519, exhibiting ligase activity in the presence of magnesium ions in a wide pH range 7,0-10,7 and 25-150 mM sodium chloride concentrations, consisting of 559 amino acids, having a weight of 63 kD as determined by gel electrophoresis and polyacrylic comprising the amino acid sequence of SEQ ID NO: 2, obtainable from recombinant E. coli strain. ! 2. An isolated nucleotide sequence of DNA encoding a thermostable DNA ligase according to claim 1, comprising the nucleotide sequence of SEQ ID NO: 1. ! 3. A process for preparing a thermostable DNA ligase according to claim 1, comprising culturing the recombinant strain of E.coli DLT1270, containing plasmid pRARE, transformed vector pQE30, comprising a nucleotide sequence of SEQ ID NO: 1 encoding DNA ligase Thermococcus sp. 1519, isolation and DNA ligase purification by metal-affinity chromatography.