METHOD OF STUDYING MIGRATION OF STEM CELLS IN VITRO AND PRODUCTIONS OF HOMING FACTORS OF PROGENITOR ELEMENTS BY CELLS OF TISSUES MICROENVIRONMENT

摘要

FIELD: medicine.;SUBSTANCE: cell material containing marrow stem cells in a liquid culture medium is placed over a prepared agar medium containing marrow cells producing homing factors. After incubation of the prepared two-layer culture, inadherent cells are transferred to a semi-viscous culture medium and incubated in a mode required for colony-formation. Then by recording the difference of stem cell count in the cell material in the reference, and after placing on the agar medium containing homing factors, stem cells migrated by stem cell homing factor are counted.;EFFECT: invention allows simplifying the method for determination of production of stem cell homing factors due to the use of the agar medium as a semipermeable membrane and the introduction of colony-forming ability change of the cell material as an evaluation criterion of production of stem cell homing factors.;1 tbl, 1 ex