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METHOD FOR LILIES REPRODUCTION IN VITRO

机译:百合的体外繁殖方法

摘要

FIELD: agriculture.;SUBSTANCE: invention relates to the field of gardening. In method explants are separated, treated with a solution of epibrassinolid in concentration of 0.1 mg/l and time of exposition of 1 hr, exposed to sterilisation. Then explants are planted into agarised nutrient medium of Murasige and Skoog with content of growth regulators - 1.5 mg/l of 6-benzylaminopurine and 0.5 mg/l of indoleacetic acid. Then microbulbs are separated from explants and implanted into nutrient medium of Murasige-Skoog with addition of 10-11 mole/l of tidiazuron and 0.5 mg/l of a-naphthyl acetic acid in darkness at the temperature of 14°C.;EFFECT: invention makes it possible to increase yield of reproduced material by increasing number of generated microbulbs at positive effect of optimal concentrations of growth regulators.;5 tbl
机译:技术领域:本发明涉及园艺领域。在该方法中,分离外植体,用浓度为0.1 mg / l的表油菜素内酯溶液处理,暴露时间为1小时,并进行灭菌处理。然后将外植体种植在具有生长调节剂含量的Murasige和Skoog的琼脂营养培养基中-1.5 mg / l的6-苄氨基嘌呤和0.5 mg / l的吲哚乙酸。然后将微球从外植体中分离出来,并在室温下,在黑暗中,在室温下添加10 -11 摩尔/升替地龙和0.5 mg / lα-萘乙酸,注入Murasige-Skoog营养培养基中。 14°C;效果:本发明可以通过在最佳浓度的生长调节剂的积极作用下增加产生的微球的数量来提高再生材料的产量.5 tbl

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